Department of Microbiology

Strona www

Head

Professor Jan Potempa, PhD DSc
room: B220 (3.1.24), phone: +48 12 664 63 43

Staff

Jacek Międzobrodzki, PhD, DSc, room: C242 (3.1.32), phone: +48 12 664 63 71
Joanna Kozieł, PhD, DSc, room: B219 (3.1.23), phone: +48 12 664 63 77
Maciej Lech, PhD, DSc, room: B219 (3.1.23), phone: +48 12 664 63 77
Krzysztof Pyrć, PhD, DSc, room: C225 (2.1.29), phone: +48 12 664 61 21, http://virogenetics.info/
Grzegorz Dubin, PhD, DSc, room: B204 (3.1.36), phone: +48 12 664 63 62
Piotr Mydel, PhD, DSc, room: B203 (3.1.37), phone: +48 12 664 63 78
Dariusz Dziga, PhD, DScroom: B243 (3.1.32), phone: 12 664 65 40

Anna Maksylewicz, PhD, Eng, room: B243 (3.1.32), phone: 12 664 65 40
Kinga Wójcik, PhD, room: B201 (3.1.38), phone: +48 12 664 61 68
Danuta Mizgalska, PhD, room: B219 (3.1.23), phone: +48 12 664 63 77
Mirosław Książek, PhD, room: C241 (2.1.33), phone: +48 12 664 62 43 
Marcin Wasylewski, PhD, room: C241 (2.1.33), phone: +48 12 664 62 43
Małgorzata Benedyk, PhD, room: C243 (2.1.31), phone: +48 12 664 61 29
Danuta Bryzek, PhD, room: C243 (2.1.31), phone: +48 12 664 61 29
Katarzyna Gawron, PhD, room: C243 (2.1.31), phone: +48 12 664 61 29
Magdalena Kalińska, PhD, room: C241 (2.1.33), phone: +48 12 664 62 43
Przemysław Grudnik, PhD, room: B204 (3.1.36), phone: +48 12 664 63 62
Krzysztof Rembacz, PhD, room: B204 (3.1.36), phone: +48 12 664 63 62

Karina Adamowicz, MSc, room: B219 (3.1.23), phone: +48 12 664 63 77
Aleksandra Milewska, MSc, room: C241 (2.1.33), phone: +48 12 664 62 43
Ewa Bielecka, MSc, room: B219 (3.1.23), phone: +48 12 664 63 77
Dominika Staniec, MSc, room: C243 (2.1.31), phone: +48 12 664 61 29
Katherine Falkowski, MSc, room: C241 (2.1.33), phone: +48 12 664 62 43
Laura Sąsiadek, MSc, room: C241 (2.1.33), phone: +48 12 664 62 43
Marta Kossowicz-Reroń, MSc, room: B203 (3.1.37), phone: +48 12 664 63 78
Magdalena Nowak, MSc, room: B219 (3.1.23), phone: +48 12 664 63 77
Agata Potempa, MSc, room: B203 (3.1.37), phone: +48 12 664 63 78
Aneta Sroka, MSc, room: B203 (3.1.37), phone: +48 12 664 63 78
Alicia Wong, MSc, room: B219 (3.1.23), phone: +48 12 664 63 77
Valeria Napolitano, MSc, room: C241 (2.1.33), phone: +48 12 664 62 43

PhD students

Ewa Bielecka, room: B219 (3.1.23), phone: +48 12 664 63 77
Józefina Bogusz, room: C241 (2.1.33), phone: +48 12 664 62 43
Aneta Buda, room: B205 (3.1.35), phone: +48 12 664 63 65
Michał Burmistrz, room: C241 (2.1.33), phone: +48 12 664 62 43
Ewelina Dobosz, room: C243 (2.1.31), phone: +48 12 664 61 29
Izabela Główczyk, room: C241 (2.1.33), phone: +48 12 664 62 43
Przemysław Golik, room: C241 (2.1.33), phone: +48 12 664 62 43
Maja Kosecka, room: B205 (3.1.35), phone: +48 12 664 63 65
Katarzyna Kosowicz, room: C241 (2.1.33), phone: +48 12 664 62 43
Aleksandra Milewska, room: C241 (2.1.33), phone: +48 12 664 62 43
Artur Szczepański, room: C241 (2.1.33), phone: +48 12 664 62 43
Magdalena Widziołek, room: C241 (2.1.33), phone: +48 12 664 62 43
Karol Źrubek, room: C241 (2.1.33), phone: +48 12 664 62 43
Zuzanna Nowakowska, room: C243 (2.1.31), phone: +48 12 664 61 29
Mariusz Madej, room: C241 (2.1.33), phone: +48 12 664 62 43
Małgorzata Magoch, room: C241 (2.1.33), phone: +48 12 664 62 43
Anna Marzęda, room: C241 (2.1.33), phone: +48 12 664 62 43
Marta Kamińska, room: C241 (2.1.33), phone: +48 12 664 62 43
Agata Marczyk, room: C241 (2.1.33), phone: +48 12 664 62 43
Robert Paczyński, room: C241 (2.1.33), phone: +48 12 664 62 43
Aleksandra Pęcak, room: C241 (2.1.33), phone: +48 12 664 62 43
Bożena Szelążek, room: C241 (2.1.33), phone: +48 12 664 62 43
Natalia Stach, room: C241 (2.1.33), phone: +48 12 664 62 43
Klaudia Lisowska, room: B205 (3.1.35), phone: +48 12 664 63 65
Agata Wardalska, room: B205 (3.1.35), phone: +48 12 664 63 65
Magdalena Pachota, room: C241 (2.1.33), phone: +48 12 664 62 43

Research topics

  • Pathogenesis of autoimmune diseases, including rheumatoid arthritis
  • Role of posttranslational modifications in immune system regulation
  • Role of negative inflammatory regulators in bacterial infection
  • Role of kallikrein-related proteases in EGF-related signal transduction
  • Role of arginine citrullination in the protease-inhibitor interactions in the context of innate immunity system
  • Sigficance of bacterial citrullination of host proteins/peptides in pathogenesis of periodontal disease
  • Role of citrullination in healing and regeneration of periodontal tissues
  • Petiopathogenesis and novel approaches to therapy of gingival fibromatosis
  • Mechanisms of endocytic entry of human viruses
  • Virus-receptor interactions
  • Defense mechanisms of bacteria
    Epigenetics of bacterial infections

Methods

  • Flow cytometry
  • Fluorescence microscopy
  • Gene expression analysis (qPCR)
  • Protein analysis (ELISA, Western Blot)
  • DNA fragmentation analysis
  • Cell death analysis, cytotoxicity, cell proliferation, differentiation
  • Primary tissue cultures/organotypic cultures of gingival mucosa
  • Cell infection methods
  • Histological staining, immunohistochemistry, tissue analysis (computer analyses)
  • Analiza białek, HPLC chromatography
  • Animal models of infection

Current projects

  1. Aleksander Grabiec: Epigenetics of periodontitis: alterations in the host protein acetylation system as a potentially fundamental mechanism for disease development. (2016-2018). POLONEZ 1, National Science Centre (NCN).
  2. Danuta Mizgalska: Application of SELEX methodology for selection of aptamers as research and diagnostic tools to study and potentially treat periodontitis. (2015-2018). SONATA 8, National Science Centre (NCN).
  3. Krzysztof Pyrć: Coronavirus and the cell: dyplomatic bag. (2013-2018). SONATA BIS, National Science Centre (NCN).
  4. Jan Potempa: Periodontopathogens as ethiologic factors of systemic diseses. (2014-2017). Ministry of Science and Higher Education.
  5. Jan Potempa: King of hearts, joints and lungs: periodontal pathogens as etiologic factor in RA, CVD and COPD and their impact on treatment strategies. (2013-2017). 7th Framework Program.
  6. Jan Potempa: Extracellular proteolytic activity of human epithelium – role in the modulation of signal transduction. (2013-2017), SYMFONIA 1, National Science Centre (NCN).
  7. Grzegorz Dubin:  Kinases of emerging relevance in cancer – structural characterization. (2013-2017), SONATA BIS, National Science Centre (NCN).
  8. Jan Potempa: Unique secretion system of periodontopathogens. (2012-2017), MAESTRO 2, National Science Centre (NCN).
  9. Przemysław Golik: Biochemical and structural characterization of a protein involved in a sporulation YabG proteinase. (2015-2016). ETIUDA 3, National Science Centre (NCN).
  10. Katarzyna Gawron: Significance of peptides citrullinated by peptidylarginine deiminase from Porphyromonas gingivalisin periodontitis. (2013-2016). OPUS 4, National Science Centre (NCN).
  11. Aleksandra Milewska: Identification of the APOBEC3 cytidine deaminases as an additional human defense system against RNA viruses, on the example of coronaviruses. (2013-2016). PRELUDIUM 4, National Science Centre (NCN).
  12. Joanna Kozieł: The role of MCPIP1 in the regulation of innate immunity during infection. (2012-2016). OPUS 2, National Science Centre (NCN).
  13. Krzysztof Pyrć: NCN Porphyromonas gingivalis: natural defense and regulatory mechanism. (2011-2016). SONATA 1, National Science Centre (NCN).

Selected publications

  1. de Diego I, Ksiazek M, Mizgalska D, Koneru L, Golik P, Szmigielski B, Nowak M, Nowakowska Z, Potempa B, Houston JA, Enghild JJ, Thøgersen IB, Gao J, Kwan AH, Trewhella J, Dubin G, Gomis-Rüth FX, Nguyen KA, Potempa J. The outer-membrane export signal of Porphyromonas gingivalis type IX secretion system (T9SS) is a conserved C-terminal β-sandwich domain. Sci Rep. 2016;6:23123.
  2. Benedyk M, Mydel PM, Delaleu N, Płaza K, Gawron K, Milewska A, Maresz K, Koziel J, Pyrc K, Potempa J. Gingipains: Critical factors in the development of aspiration pneumonia caused by Porphyromonas gingivalis. J Innate Immun. 2016;8(2):185-98.
  3. Laugisch O, Wong A, Sroka A, Kantyka T, Koziel J, Neuhaus K, Sculean A, Venables PJ, Potempa J, Möller B, Eick S. Citrullination in the periodontium – a possible link between periodontitis and rheumatoid arthritis. Clin Oral Investig. 2016;20(4):675-83.
  4. Staniec D, Ksiazek M, Thøgersen IB, Enghild JJ, Sroka A, Bryzek D, Bogyo M, Abrahamson M, Potempa J. Calcium regulates the activity and structural stability of Tpr, a bacterial calpain-like peptidase. J Biol Chem. 2015;290(45):27248-60.
  5. Burmistrz M, Dudek B, Staniec D, Rodriguez Martinez JI, Bochtler M, Potempa J, Pyrc K. Functional Analysis of Porphyromonas gingivalis W83 CRISPR-Cas Systems. J Bacteriol. 2015;197(16):2631-41.
  6. Ksiazek M, Mizgalska D, Enghild JJ, Scavenius C, Thogersen IB, Potempa J. Miropin, a novel bacterial serpin from the periodontopathogen Tannerella forsythia, inhibits a broad range of proteases by using different peptide bonds within the reactive center loop. J Biol Chem. 2015;290(1):658-70.
  7. Koziel J, Chmiest D, Bryzek D, Kmiecik K, Mizgalska D, Maciag-Gudowska A, Shaw LN, Potempa J. The Janus face of α-toxin: a potent mediator of cytoprotection in staphylococci-infected macrophages. J Innate Immun. 2015;7(2):187-98.
  8. Gawron K, Bereta G, Nowakowska Z, Lazarz-Bartyzel K, Lazarz M, Szmigielski B, Mizgalska D, Buda A, Koziel J, Oruba Z, Chomyszyn-Gajewska M, Potempa J. Peptidylarginine deiminase from Porphyromonas gingivalis contributes to infection of gingival fibroblasts and induction of prostaglandin E2-signaling pathway. Mol Oral Microbiol. 2014;29(6):321-32.
  9. Koziel J, Bryzek D, Sroka A, Maresz K, Glowczyk I, Bielecka E, Kantyka T, Pyrć K, Svoboda P, Pohl J, Potempa J. Citrullination alters mmunomodulatory function of LL-37 essential for prevention of endotoxin-induced sepsis. J Immunol. 2014;192(11):5363-72.
  10. Pustelny K, Zdzalik M, Stach N, Stec-Niemczyk J, Cichon P, Czarna A, Popowicz G, Mak P, Drag M, Salvesen GS, Wladyka B, Potempa J, Dubin A, Dubin G. Staphylococcal SplB serine protease utilizes a novel molecular mechanism of activation. J Biol Chem. 2014;289(22):15544-53.

Batchelor/master thesis topics

  • Identification of bacterial and virial virulence factors
  • Modulation of immunological response during infection
  • Potranslational protein modifications during inflammation

Requirements for candidates

  • High score in major cell biology/biochemistry and/or microbiology courses
  • Techniques: basic biochemical methods, bacterial cultivation