Department of Immunology

Head

Professor Joanna Cichy, PhD, DSc
room: C220 (2.1.24), phone: +48 12 664 61 27, e-mail: joanna.cichy@uj.edu.pl

Staff

Małgorzata Bzowska, PhD
room: C222 (2.1.26), phone: +48 12 664 61 58, e-mail: malgorzata.bzowska@uj.edu.pl

Krzysztof Guzik, PhD
room: C223 (2.1.27), phone: +48 12 664 61 37, e-mail: krzysztof.guzik@uj.edu.pl

Mateusz Kwitniewski, PhD
room: C220 (2.1.24), phone: +48 12 664 61 35, e-mail: mateusz.kwitniewski@uj.edu.pl

Joanna Skrzeczyńska–Moncznik, PhD
room: C222 (2.1.26), phone: +48 48 12 664 61 58, e-mail: j.skrzeczynska.moncznik@uj.edu.pl

Katarzyna Bulek, PhD
room: C221 (2.1.25), phone: +48 12 664 62 01, e-mail: katarzyna.bulek@uj.edu.pl

Małgorzata Bodaszewska-Lubaś, PhD
room: C221 (2.1.25), phone: +48 12 664 62 01, e-mail: malgorzata.bodaszewska@uj.edu.pl

Beata Grygier, PhD
room: C220 (2.1.24), phone: +48 12 664 61 35, e-mail: beata.grygier@uj.edu.pl

Patrycja Kwiecińska, PhD
room: C220 (2.1.24), phone: +48 12 664 61 35, e-mail: patrycja.kwiecinska@uj.edu.pl

Paweł Majewski, PhD
room: C220 (2.1.24), phone: +4812 664 61 35, e-mail: pawel.majewski@uj.edu.pl

Anna Sanecka-Duin, PhD
room: C221 (2.1.25), phone: +48 12 664 62 01, e-mail: anna.sanecka-duin@uj.edu.pl

Katarzyna Szmyd, PhD
room: C219 (2.1.23), phone: +48 12 664 61 26, e-mail: katarzyna.szmyd@uj.edu.pl

Monika Majchrzak-Górecka, MSc
room: C221, (2.1.25), phone: +48 12 664 62 01

Joanna Marczyńska, PhD
room: C221 (2.1.25), phone: +48 12 664 62 01, e-mail: joanna.marczynska@uj.edu.pl

Anna Piszczek, MSc
room: C223, (2.1.28), phone: +48 12 664 61 61, e-mail: anna.piszczek@uj.edu.pl

Alicja Stec, MSc
room: C223, (2.1.28), phone: +48 12 664 61 61, e-mail: alicja.stec@uj.edu.pl

Aneta Zegar, MSc
room: C221, (2.1.25), phone: +48 12 664 62 01, e-mail: aneta.zegar@uj.edu.pl

PhD students

Piotr Brzoza, room: C223 (2.1.28), phone: +48 12 664 61 61
Kamila Kwiecień, room: C223 (2.1.28), phone: +48 12 664 61 61
Urszula Godlewska, room: C223 (2.1.28), phone: +48 12 664 61 61
Monika Majchrzak-Górecka, room: C221 (2.1.25),phone: +48 12 664 62 01
Agnieszka Morytkoroom: C223 (2.1.28), phone: +48 12 664 61 61
Oktawia Osiecka, room: C223 (2.1.28), phone: +48 12 664 61 61
Aneta Zegar, room: C221 (2.1.25), phone: +48 12 664 62 01

Research topics

  • The role of proteinases and their inhibitors in regulation of immune responses
  • Pathogenesis of autoimmune diseases, including skin disease psoriasis
  • Mechanisms underlying formation and inhibition of neutrophil extracellular traps (NETs)
  • The role of chemoattractant protein-chemerin in immune and metabolic responses
  • Mechanisms of recognition of dying cells by professional phagocytes
  • The role of 27kDa Heat Shock Protein (HSP27) in the formation of damage-associated molecular patterns (DAMPs)
  • The role of cell surface 90kDa Heat Shock Protein (HSP90) in the recognition of molecular patterns
  • The influence of low-density oxidised lipoproteins (oxLDL) on the recognition of molecular patterns by professional phagocytes

Methods and specialistic equipment

  • Flow cytometry and cell sorting (LSRII, 16 colors; automatic magnetic cell sorter)
  • Fluorescence microscopy (fully-motorized NIKON Eclipse microscope)
  • Gene expression analysis (qPCR)
  • Protein analysis (ELISA, Western Blot, Multiplex)
  • Metabolic analysis (Seahorse XFp)
  • Production of mutant proteins
  • Analysis of DNA metylation
  • Isolation and tissue culture of primary cells (keratinocytes, adipocytes, hepatocytes)
  • 3D cultures of keratinocytes
  • Experimental mouse models (psoriasis model, SLPI, Tig2 and CMKLR1 KO mice)

Current projects

  1. Beata Grygier: SLPI – novel regulator involved in the neuro-immune interplay in psoriasis (2017-2020). SONATA 12, National Science Centre (NCN).
  2. Katarzyna Bulek: Mechanism of epigenetic-driven inactivation of tumor suppressor SIGIRR (2016-2019). OPUS 10, National Science Centre (NCN).
  3. Joanna Cichy: Role of antibacterial chemerin in skin pathophysiology. (2014-2019). SYMFONIA 2, National Science Centre (NCN).
  4. Mateusz Kwitniewski: Novel factors and signaling pathways regulating expression of chemerin. (2014-2018). SONATA BIS 3, National Science Centre (NCN).
  5. Joanna Cichy: The role of inhibitors of serine proteases in regulation of dendritic cell function. (2012-2017). MAESTRO 2, National Science Centre (NCN).

Selected publications

  1. Godlewska U, Brzoza P, Sroka A, Majewski P, Jentsch H, Eckert M, Eick S, Potempa J, Zabel BA, Cichy J. Antimicrobial and Attractant Roles for Chemerin in the Oral Cavity during Inflammatory Gum Disease. Front Immunol2017 Mar 29;8:353. eCollection 2017.
  2. Majchrzak-Gorecka M, Majewski P, Grygier B, Murzyn K, Cichy J.Secretory leukocyte protease inhibitor (SLPI), a multifunctional protein in the host defense response. Cytokine Growth Factor Rev2016 Apr;28:79-93. Review
  3. Skrzeczyńska-Moncznik J., Bzowska M., Nogieć A., Sroka A., Zarębski M., Vallières L., Guzik K. Rapid externalization of 27-kDa heat shock protein (HSP27) and atypical cell death in neutrophils treated with the sphingolipid analog drug FTY720. J Leukoc Biol2015;98(4):591-9.
  4. Zabieglo K., Majewski P., Majchrzak-Gorecka M., Wlodarczyk A., Grygier B., Zegar A., Kapinska-Mrowiecka M., Naskalska A., Pyrc K., Dubin A., Wahl S.M., Cichy J. The inhibitory effect of secretory leukocyte protease inhibitor (SLPI) on formation of neutrophil extracellular traps. J Leukoc Biol2015;98(1):99-106.
  5. Marczynska J., Ozga A., Wlodarczyk A., Majchrzak-Gorecka M., Kulig P., Banas M., Michalczyk-Wetula D., Majewski P., Hutloff A., Schwarz J., Chalaris A., Scheller J., Rose-John S., Cichy J. The role of metalloproteinase ADAM17 in regulating ICOS ligand-mediated humoral immune responses. J Immunol2014;193(6):2753-63.
  6. Banas M., Zabieglo K., Kasetty G., Kapinska-Mrowiecka M., Borowczyk J., Drukala J., Murzyn K., Zabel B.A., Butcher E.C., Schroeder J.M., Schmidtchen A., Cichy J. Chemerin is an antimicrobial agent in human epidermis. PLoS One2013;8(3):e58709.
  7. Skrzeczynska-Moncznik J., Wlodarczyk A., Zabieglo K., Kapinska-Mrowiecka M., Marewicz E., Dubin A., Potempa J., Cichy J. Secretory leukocyte proteinase inhibitor-competent DNA deposits are potent stimulators of plasmacytoid dendritic cells: implication for psoriasis. J Immunol2012;189(4):1611-7.
  8. Kulig P., Kantyka T., Zabel B.A., Banas M., Chyra A., Stefanska A., Tu H., Allen S.J., Handel T.M., Kozik A., Potempa J., Butcher E.C., Cichy J. Regulation of chemerin chemoattractant and antibacterial activity by human cysteine cathepsins. J Immunol2011;187(3):1403-10.
  9. Guzik, K., Bzowska, M., Smagur, J., Krupa, O., Sieprawska, M., Travis, J., Potempa, J. A new insight into phagocytosis of apoptotic cells: proteolytic enzymes divert the recognition and clearance of polymorphonuclear leukocytes by macrophages. Cell Death Differ2007;14:171-82.
  10. Kulig, P. Zabel, B. A., Dubin, G., Allen, S. J., Ohyama, T., Potempa, J., Handel, T. M., Butcher, E. C., and Cichy, J. Staphylococcus aureus-derived staphopain B, a potent cysteine protease activator of plasma chemerin.  J. Immunol2007;178:3713-20.

Batchelor/master thesis topics

  • The role of proteinases and their inhibitors in regulation of immune responses
  • Pathogenesis of autoimmune diseases, including skin disease psoriasis
  • Mechanisms underlying formation and inhibition of neutrophil extracellular traps (NETs)
  • The role of chemoattractant protein-chemerin in immune and metabolic response

Requirements for candidates

  • Interest in experimental immunology
  • Basic knowledge in immunology,  molecular biology and cell biology 
  • Proficiency in spoken and written English