Department of Cell Biology

Webpage

Head

Professor Zbigniew Madeja, PhD, DSc
room: C120 (2.0.25), phone: +48 12 664 61 42, e-mail: z.madeja@uj.edu.pl

Staff

Prof Włodzimierz Korohoda, profesor emeritus, room: C126 (2.0.31), phone: +48 12 664 61 25, e-mail: w.korohoda@uj.edu.pl
Prof Jarosław Czyż, PhD, DSc room: C123 (2.0.28), phone: +48 12 664 61 46, e-mail: jarek.czyz@uj.edu.pl
Ewa Zuba-Surma, PhD, DSc, room: C119A (2.0.24A), phone: +48 12 664 61 80, e-mail: ewa.zuba-surma@uj.edu.pl
Justyna Drukała, PhD, DSc, room: C124 (2.0.29), phone: +48 12 664 61 45, e-mail: justyna.drukala@uj.edu.pl
Marta Michalik, PhD, DSc, room: C118 (2.0.23), phone: +48 12 664 61 44, e-mail: marta.michalik@uj.edu.pl
Jolanta Sroka, PhD, DSc, room: C121 (2.0.26), phone: +48 12 664 61 43, e-mail: jolanta.sroka@uj.edu.pl

Sylwia Bobis-Wozowicz, PhD, room: C119B (2.0.24B), phone: +48 12 664 62 27, e-mail: sylwia.bobis@uj.edu.pl
Katarzyna Piwowarczyk, PhD, room: C122 (2.0.27), phone: +48 12 664 61 83, e-mail: katarzyna.szpak@uj.edu.pl
Monika Rak, PhD, room: C122 (2.0.27), phone: +48 12 664 61 83, e-mail: monika.rak@uj.edu.pl
Damian Ryszawy, PhD, room: C125 (2.0.30), phone: +48 12 664 61 47, e-mail: damian.ryszawy@uj.edu.pl

Julia Borowczyk-Michałowska, PhD, room: C129 (2.0.7), phone: +48 12 664 61 89, e-mail: julia.borowczyk@uj.edu.pl
Milena Paw, PhD, room: C119B (2.0.24B), phone: +48 12 664 62 27, e-mail: milena.paw@uj.edu.pl
Małgorzata Sekuła, PhD, room: MCB 1/31, e-mail: malgorzata.sekula@uj.edu.pl
Eliza Zimoląg, PhD, room: C129 (2.0.7), phone: +48 12 664 61 88, e-mail: eliza.zimolag@uj.edu.pl


Lidia Ciastoń, MSc, room: C137 (2.0.13), phone: +4812 664 61 40, e-mail: lidia.ciaston@uj.edu.pl
Jadwiga Golda, MSc, room: C125 (2.0.30), phone: +48 12 664 61 47, e-mail: jadwiga.golda@uj.edu.pl
Elżbieta Karnas, MSc, room: C125 (2.0.30), phone: +48 12 664 61 47, e-mail: e.karnas@uj.edu.pl
Anna Łabędź-Masłowska, MSc, room: C122 (2.0.27), phone: +48 12 664 61 83, e-mail: anna.labedz-maslowska@uj.edu.pl
Sławomir Lasota, MSc, room:  C119B (2.0.24B), phone: +48 664 62 27, e-mail: slawomir.lasota@uj.edu.pl
Maria Starostka-Wróbel, MSc, Eng, room: C137 (2.0.13), phone: +48 12 664 61 40, e-mail: maria.starostka-wrobel@uj.edu.pl

PhD students

Marta Adamiak, room: C119B (2.0.24B), phone: +48 12 664 62 27
Elżbieta Karnas, room: C125 (2.0.30), phone: +48 12 664 61 47
Katarzyna Kmiotek, room: MCB 1/31
Marcin Luty, room:C122 (2.0.27), phone: +48 12 664 61 83
Anna Łabędź-Masłowska, room: C122 (2.0.27), phone: +48 12 664 61 83
Sylwia Noga, room: MCB 1/31
Joanna Stalińska, room: MCB 1/33
Dawid Wnuk, room: C125 (2.0.30), phone: +48 12 664 61 47
Jessica Catapano, room:  C125 (2.0.30), phone: +48 12 664 61 47
Tomasz Wróbel, room:  C119B (2.0.24B), phone: +48 12 664 62 27

Research topics

  • Functions of direct cell-cell interactions in regulating cancer cell migration activity
  • Influence of the functions of gap junctions and cell adhesion molecules on the invasiveness of cancer cells
  • Role of reactive oxygen species in regulating the migration activity and intercellular communication by gap junctions
  • Electrotaxis mechanisms
  • Epithelial–mesenchymal transition (EMT) mechanisms
  • Application of electrochemical methods in cell biology
  • Fibroblast to myofibroblast transition  (FMT) mechanisms  and  role  of FMT in bronchial wall remodeling in asthma
  • Mechanisms of endocytosis
  • Application of in vitro skin cell cultures in clinical practice, such as wound treatment and plastic surgery
  • Optimization of stem cell and progenitor cell populations for applications in experimental cardiology including pluripotent stem cells VSELs and iPS as well as mesenchymal stem cells of different antigenic phenotype
  • Use of stem cells and progenitor cells in regenerative medicine (http://www.stemcells-project.eu)
  • Cancer stem cells, cancer drug-resistance
  • Role of stem cell derived extracellular vesicles (native and genetically modified) in tissue regeneration
  • Optimization of biocompatible scaffolds combining graphene and defined stem cell populations for tissue regeneration

Methods and specialistic equipment

  • Cell and tissue engineering
  • Fluorescence microscopy
  • Flow cytometry and cell sorting
  • Water purification system Simplicity UV (Merk)
  • CFX384 Real Time System (BioRad) for gene expression analysis on 384 well plates
  • FACSAria III (Becton Dickinson) fluorescence-activated cell sorting system
  • Olympus IX81 fluorescent microscope equiped with MicroPublisher 3.3 RTV camera
  • Neon Electroporation System (Invitrogen) for cell electroporation

Current projects

  1. Katarzyna Kmiotek: Optimization of parameters for evaluation of cardiomyogenesis efficacy in human cardiac mesenchymal stromal cells, during differentiation process, after treatment with human induced pluripotent stem cell derived extracellular vesicles. (2017-2019). PRELUDIUM 11, National Science Centre (NCN).
  2. Sylwia Bobis-Wozowicz: Impact of hypoxia on molecular characteristic and biological properties, including heart regenerative capacity of extracellular vesicles released by human induced pluripotent stem cells. (2017-2020). SONATA 12. National Science Centre (NCN).
  3. Ewa Zuba-Surma: Development of optimized methods for treatment of tissue injuries based on innovative composites and mesenchymal stem cells and their derivatives in patients with civilization diseases. (2017-2020). STRATEGMED, National Centre for Research and Development (NCBiR) 
  4. Jarosław Czyż: Interference of fenofibrate with the invasive potential of prostate cancer cells modulated by chemotherapeutics. (2016-2019). OPUS 9, National Science Centre (NCN).
  5. Marta Michalik: Bronchial fibroblast to myofibroblast transition – the role of differential activation of TGF-b1/Smad signalling pathway in cells derived from asthmatic patients and non-asthmatic donors – in vitro studies in 2D, 3D models and model of the epithelial-mesenchymal trophic unit. (2016-2019). OPUS, National Science Centre (NCN).
  6. Damian Ryszawy: The role of Snail-1 and Cx43 in regulation of invasive properties of human glioblastoma multiforme. (2016-2019). SONATA 10, National Science Centre (NCN).
  7. Ewa Zuba-Surma: Optimization of biocompatible scaffolds combining graphene and defined stem cell populations for tissue regeneration. (2015-2019). SYMFONIA 3, National Science Centre (NCN).
  8. Ewa Zuba-Surma: Studies of efficacy of microvesicles derived from genetically modified stem cells as carriers for proangiogenic and cardiomyogenic miRNA. (2014-2017). SONATA BIS 3, National Science Centre (NCN).

Selected publications

  1. Borowczyk-Michalowska J., Zimolag E., Waligorska A., Dobrucki J., Madeja Z., Drukala J. Stage-specific embryonic antigen-4 as a novel marker of ductal cells of human eccrine sweat glands. Br J Dermatol. 2017; 176(6): 1541-1548. DOI: 10.1111/bjd.15154. 
  2. Piwowarczyk K, Paw M, Ryszawy D, Rutkowska-Zapała M, Madeja Z, Siedlar M, Czyż J. Connexin43high prostate cancer cells induce endothelial connexin43 up-regulation through the activation of intercellular ERK1/2-dependent signaling axis. Eur J Cell Biol. 2017 Jun;96(4):337-346. 
  3. Paw M, Borek I, Wnuk D, Ryszawy D, Piwowarczyk K, Kmiotek K, Wójcik-Pszczoła KA, Pierzchalska M, Madeja Z, Sanak M, Błyszczuk P, Michalik M, Czyż J. Connexin43 Controls the Myofibroblastic Differentiation of Bronchial Fibroblasts from Patients with Asthma. Am J Respir Cell Mol Biol. 2017 Jul;57(1):100-110.
  4. Sroka J, Krecioch I, Zimolag E, Lasota S, Rak M, Kedracka-Krok S, Borowicz P, Gajek M, Madeja Z. Lamellipodia and membrane blebs drive efficient electrotactic migration of rat Walker carcinosarcoma cells WC 256. PLoS One. 2016;11(2):e0149133.
  5. Sarna M, Wójcik K, Hermanowicz P, Wnuk D, Burda K, Sanak M, Czyż J, Michalik M. Undifferentiated bronchial fibroblasts derived from asthmatic patients display higher elastic modulus than their non-asthmatic counterparts. PlosOne. 2015
  6. Labedz-Maslowska A, Lipert B, Berdecka D, Kedracka-Krok S, Jankowska U, Kamycka E, Sekula M, Madeja Z, Dawn B, Jura J, Zuba-Surma EK. Monocyte chemoattractant protein-induced protein 1 (MCPIP1) enhances angiogenic and cardiomyogenic potential of murine bone marrow-derived mesenchymal stem cells. PLoS One. 2015;10(7):e0133746.
  7. Piwowarczyk K, Wybieralska E, Baran J, Borowczyk J, Rybak P, Kosińska M, Włodarczyk AJ, Michalik M, Siedlar M, Madeja Z, Dobrucki J, Reiss K, Czyż J. Fenofibrate enhances barrier function of endothelial continuum within the metastatic niche of prostate cancer cells. Expert Opin Ther Targets. 2015;19(2):163-76.  
  8. Bobis-Wozowicz S, Kmiotek K, Sekula M, Kedracka-Krok S, Kamycka E, Adamiak M, Jankowska U, Madetko-Talowska A, Sarna M, Bik-Multanowski M, Kolcz J, Boruczkowski D, Madeja Z, Dawn B, Zuba-Surma EK. Human induced pluripotent stem cell-derived microvesicles transmit RNAs and proteins to recipient mature heart cells Modulating cell fate and behavior. Stem Cells. 2015;33(9):2748-61.
  9. Kozdęba M, Borowczyk J, Zimoląg E, Wasylewski M, Dziga D, Madeja Z, Drukala J. Microcystin-LR affects properties of human epidermal skin cells crucial for regenerative processes. Toxicon. 2014;80:38-46.
  10. Ryszawy D, Sarna M, Rak M, Szpak K, Kędracka-Krok S, Michalik M, Siedlar M, Zuba-Surma E, Burda K, Korohoda W, Madeja Z, Czyż J. Functional links between Snail-1 and Cx43 account for the recruitment of Cx43-positive cells into the invasive front of prostate cancer. Carcinogenesis. 2014;35(9):1920-30.
  11. Michalik M, Pierzchalska M, Włodarczyk A, Wójcik KA, Czyż J, Sanak M, Madeja Z. Transition of asthmatic bronchial fibroblasts to myofibroblasts is inhibited by cell-cell contacts. Respir Med. 2011;105(10):1467-75.
  12. Sroka J, Antosik A, Czyz J, Nalvarte I, Olsson JM, Spyrou G, Madeja Z. Overexpression of thioredoxin reductase 1 inhibits migration of HEK-293 cells. Biol Cell. 2007;99(12):677-87.

Batchelor/master thesis topics

  • Use of stem cells and progenitor cells in regenerative medicine (http://www.stemcells-project.eu/)
  • Role of stem cell derived extracellular vesicles (native and genetically modified) in tissue regeneration
  • Optimization of biocompatible scaffolds combining graphene and defined stem cell populations for tissue regeneration
  • Interaction between tumor cells and normal tissue: morphology, motility and cell-cell interaction between tumor cells and normal cells
  • Influence of cell-cell interactions on the activity of tumor cell migration
  • Modifying agents of cytoskeletal organization normal and neoplastic cells
  • Galvanotaxis and chemotaxis of various cell
  • Mechanisms of endocytosis
  • Intercellular communication through gap junctions
  • Mechanisms of cancer progression
  • Electrochemical methods in cell biology: cell separation, elec troperforation and electrofusion
  • The use of human cells cultured in vitro for transplantology
  • iPS- and MSC- derived extracellular vesicles as a promissing tool in in tissue regeneration
  • Impact of human extracellular vesicles derived from genetically modified stem cells on angigenic and cardiomyogenic potential of cardiac cells
  • Optimization of application of innovative biocompatible scaffolds and stem cells in tissue regeneration

Requirements for candidates

Basic knowledge on cell biology and molecular biology techniques – cell cultures and microscopy