Department of Biophysics

Department of Biophysics consists of two laboratories:

  • Laboratory for Cancer Radiospectroscopy and Radiobiology
  • Laboratory of Photobiophysics

Head

Professor Krystyna Urbańska, PhD, DSc
room: A028 (4.01.27), phone: + 48 12 664 61 53

Staff

Laboratory for Cancer Radiospectroscopy and Radiobiology:
Martyna Elas, PhD, DSc, room: B047 (3.01.37), phone: + 48 12 664 63 38
Przemysław M. Płonka, PhD, DSc, room: C026 (2.01.27), phone: + 48 12 664 63 50
Beata Płonka, PhD, room: C026 (2.01.27), phone: + 48: 12 664 63 50
Martyna Krzykawska-Serda, PhD, room: B046 (3.01.38), phone: + 48 12 664 64 42
Michał Sarna, PhD, room: C027 (2.01.28), phone: + 48 12 664 61 50
Małgorzata Szczygieł, PhD, room: C028 (2.01.29), phone: + 48 12 664 64 30
Dominika Michalczyk-Wetula, MSc, room: C028 (2.01.29), phone: + 48 12 664 64 30

Laboratory of Photobiophysics:
professor Tadeusz Sarna, PhD, DSc, head of Laboratory
room: A038 (3.01.32), phone: + 12 664 64 27

professor Witold Korytowski, PhD, DSc, room: A005 (4.01.28), phone: + 48 12 664 65 17
Anna Wiśniewska-Becker, PhD, DSc, room: B001 (3.01.43), phone: + 48 12 664 63 55
Agnieszka Wolnicka-Głubisz, PhD, DSc, room: A004 (4.01.29), phone: + 48 12 664 65 26
Gregor Becker, PhD, room: B001 (3.01.43), phone: + 48 12 664 63 55
Tomasz Panz, PhD, room: A003 (4.01.30), phone: + 48 12 664 65 14
Anna Pawlak, PhD, room: B021 (3.01.29), phone: + 48 12 664 64 25
Andrzej Żądło, PhD, room: B045 (3.01.39), phone: + 48 12 664 63 42
Anna Piłat, PhD, room: B021 (3.01.29), phone: + 48 12 664 64 25
Agnieszka Broniec, PhD, room: A004 (4.01.29), phone: + 48 12 664 65 26
Magdalena Olchawa, PhD, room: C027 (2.01.28), phone: + 48 12 664 61 50
Artur Sawicki, PhD, room: A010 (4.01.2), phone: + 48 12 664 65 73
Grzegorz Szewczyk, PhD, room: B044 (3.01.40), phone: + 48 12 664 64 40
Barbara Czuba-Pełech, Eng, room: A001 (4.01.31), phone: + 48 12 664 65 29
Grażyna Żmuda, room: A001 (4.01.31), phone: + 48 12 664 65 29

PhD students

Laboratory for Cancer Radiospectroscopy and Radiobiology:
Agnieszka Drzał, room: B014 (3.01.5), phone: + 48 12 664 64 28
Grzegorz Gawlik, room: B014 (3.01.5), phone: + 48 12 664 64 28
Michał Gonet, room: B046 (3.01.38), phone: + 48 12 664 64 42
Katarzyna Jasińska-Konior, room: B046 (3.01.38), phone: + 48 12 664 64 42
Ewa Kowolik, room: B014 (3.01.5), phone: + 48 12 664 64 28
Marcin Markiewicz, room: B014 (3.01.5), phone: + 48 12 664 64 28
Ewa Podgórska, room: B014 (3.01.5), phone: + 48 12 664 64 28
Martyna Śniegocka, room: B014 (3.01.5), phone: + 48 12 664 64 28
Justyna Wieczorek, room: B014 (3.01.5), phone: + 48 12 664 64 28

Laboratory of Photobiophysics:
Jerzy Bazak, room: B033 (3.01.11), phone: + 48 12 664 64 26
Mariusz Duda, room: B044 (3.01.40), phone: + 48 12 664 64 40
Justyna Furso, room: B014 (3.01.5), phone: + 48 12 664 64 28
Krzysztof  Klimkiewicz, room: B014 (3.01.5), phone: + 48 12 664 64 28
Olga Krzysztyńska-Kuleta, room: C027 (3.01.28), phone: + 48 12 664 61 50
Paweł Pabisz, room: B033 (3.01.11), phone: + 48 12 664 64 26
Marta Smejda, room: B014 (3.01.5), phone: + 48 12 664 64 28
Piotr Stępień, room: B014 (3.01.5), phone: + 48 12 664 64 28
Katarzyna Wawak, room: B033 (3.01.11), phone: + 48 12 664 64 26

Research topics

Laboratory for Cancer Radiospectroscopy and Radiobiology:

  • Radiobiology
  • Phototherapy
  • Anti-cancer therapies
  • Melanogenesis
  • Cancer heterogeneity
  • Hair cycle
  • Oxidative stress and nitric oxide
  • Slime moulds
  • Modelling of biological processes
  • Bioethics

Laboratory of Photobiophysics:

  • Oxidative stress in cells structure and function
  • role of melanin and caretenoids in photoprotection
  • role of photodynamic reaction in retinal damage
  • antioxidant properties of plasmalogens
  • liposomes as photosensitizer carriers in photodynamic therapy,
  • the oxidative stress on membrane properties of antioxidants
  • Mechanisms of photodynamic killing of the cells in vitro
  • Molecular mechanisms of photoaging of the skin and eye
  • Phototoinduced  oxidative modification of  macromolecules
  • Role of MCPIP1 in melanoma
  • Effect of lysenin on model membranes
     

Methods

Laboratory for Cancer Radiospectroscopy and Radiobiology:

  • Electron paramagnetic resonance spectroscopy and imaging
  • Ultrasound imaging
  • Histology and immunohistochemistry
  • Cell culture
  • Molecular biology methods
  • Atomic force microscopy
  • Computer modelling

Laboratory of Photobiophysics:

  • Cell culture (normal and tumor)
  • Exposition to different light sources in the range of UV-VIS
  • Molecular biology methods
  • Electron paramagnetic resonance (EPR)
  • Microscopy and flow cytometry
  • Direct detection of singlet oxygen via its phosphorescence
  • Flash-photolysis
     

Current projects

Laboratory for Cancer Radiospectroscopy and Radiobiology:

  1. Martyna Elas: UM CURE 2020 New therapies for uveal melanoma. (2016-2020). Horizon 2020.
  2. Martyna Elas: Ultrasound-sensitive oxygen nanobubbles: application in cancer therapy and electron paramagnetic resonance imaging. (2016-2018). OPUS 9, National Science Centre (NCN).
  3. Krystyna Urbańska: PHOTOBRAIN:  Theranostic nanoparticles AGuIX for interstitial anti-angiogenic photodynamic therapy of glioma. (2015-2018). EuroNanoMed, The National Centre for Research and Development (NCBiR).
  4. Krystyna Urbańska: Proton beam irradiation inhibits metastasis – in search for the mechanisms. (2012-2016). OPUS 4, National Science Centre (NCN).

Laboratory of Photobiophysics:

  1. Tadeusz Sarna: The effect of low-molecular-weight antioxidants on photoreactivity of the age pigment lipofuscin and melanolipofuscin and their capacity to induce oxidative stress in retinal
    pigment epithelium. (2013-2018). MAESTRO 4, National Science Centre (NCN).
  2. Tadeusz Sarna: The dual role of the blue light – an interdisciplinary study on effects of the short
    wavelength visible light on circadian regulation, neural aspects of cognitive and
    effective functioning, and on the light contribution to degeneration and pathologies of
    retina. (2013-2018). SYMFONIA, National Science Centre (NCN).
  3. Witold Korytowski: Cholesterol hydroperoxides – Impaired steroidogenesis under oxidative stress. (2015-2017). OPUS 7, National Science Centre (NCN).
  4. Agnieszka Wolnicka-Głubisz: Role of MCPIP1 in toxicity in vitro induced by UVB and UVA radiation in keratynocytes. (2013-2017). OPUS 3, National Science Centre (NCN).
  5. Anna Pawlak: Role of oxidation products of polyunsaturated fatty acids in retinal cells photodamage and contribution of plasmalogens to protection of the retina against oxidative stress. (2013-2016). SONATA BIS, National Science Centre (NCN).

Selected publications

Laboratory for Cancer Radiospectroscopy and Radiobiology:

  1. S. Kędracka-Krok, U. Jankowska, M. Elas, U. Sowa, P. Olko, B. Romanowska-Dixon, K. Urbańska, Proteomic analysis of proton beam irradiated human melanoma cells. PLOS ONE. 2014;9(1):e84621.
  2. M. Krzykawska, J. M. Dąbrowski, L. G. Arnaut, M. Szczygieł, K. Urbańska, G. Stochel,  M. Elas, The role of strong hypoxia in tumors after treatment in the outcome of bacteriochlorin-based photodynamic therapy. Free Radic Biol Med. 2014;73:239-51
  3. M. Jakubowska, D. Michalczyk-Wetula, J. Pyka, A. Susz, K. Urbanska, B.K. Plonka, P. Kuleta, P. Lacki, M. Krzykawska-Serda, L. Fiedor, P.M. Plonka, Nitrosylhemoglobin in photodynamically stressed human tumors growing in nude mice. Nitric Oxide. 2013;35:79–88.
  4. M. Elas, J. M. Magwood, B. Butler, C. Li, R. Wardak, E. D. Barth, B. Epel, S. Rubinstein, C. A. Pelizzari, R. R. Weichselbaum, H. J. Halpern, EPR oxygen images predict tumor control by a 50 percent tumor control radiation dose. Can Res. 2013;73(17):5328-35.
  5. A. Wolnicka-Glubisz, A. Pecio, D. Podkowa, P.M. Plonka, M. Grabacka, HGF/SF increases number of skin melanocytes but does not alter quality or quantity of follicular melanogenesis. PLOS ONE. 2013;8:e74883.
  6. T. Arodź, P.M. Płonka, Effects of point mutations on protein structure are nonexponentially distributed. Proteins. 2012;80:1780–1790.
  7. M. Elas, D. Hleihel, E. Barth, C. R. Haney, K.H. Ahn, C. A. Pelizzari,  B. Epel,   R. R. Weichselbaum, H. J. Halpern: Where it's at really matters: In situ in vivo vascular endothelial growth factor spatially correlates with electron paramagnetic resonance pO2 images in tumors of living mice. Mol Imag Biol. 2011;13(6):1107-13.
  8. A. Krzywda, E. Petelenz, D. Michalczyk, P.M. Plonka, Sclerotia of the acellular (true) slime mould Fuligo septica as a model to study melanization and anabiosis. Cell Mol Biol Lett. 2008;13(1):130-143.
  9. M. Elas, R. Bell, D. Hleihel, E.B. Barth, C. McFaul, C.R. Haney, J. Bielanska, K. Pustelny, K.H. Ahn, C. A. Pelizzari, H.J. Halpern. Electron paramagnetic resonance (EPR) oxygen image hypoxic fraction plus radiation dose strongly correlates with tumor cure in FSA fibrosarcomas. Int J Rad Oncol Biol Phys. 2008;71(2): 542-549.
  10. M. Elas, J. Bielanska, K. Pustelny, L. Drelicharz, T. Skorka,U. Tyrankiewicz, M. Wozniak, S. Heinze-Paluchowska, M.Walski, L. Wojnar, P. M. Plonka  and S. Chlopicki. Detection of mitochondrial dysfunction by EPR technique in mice model of dilated cardiomyopathy. Free Radical Biology and Medicine. 2008;45:321-328.

Laboratory of Photobiophysics:

  1. Olchawa MM, Pilat AK, Szewczyk GM, Sarna TJ. Inhibition of phagocytic activity of ARPE-19 cells by free radical mediated oxidative stress. Free Radic Res. 2016;25:1-31.
  2. Ito S, Kikuta M, Koike S, Szewczyk G, Sarna M, Zadlo A, Sarna T, Wakamatsu K. Roles of reactive oxygen species in UVA-induced oxidation of 5,6-dihydroxyindole-2-carboxylic acid-melanin as studied by differential spectrophotometric method. Pigment Cell Melanoma Res. 2016;29(3):340-51.
  3. Stepien P, Polit A, Wisniewska-Becker A. Comparative EPR studies on lipid bilayer properties in nanodiscs and liposomes. Biochimica Et Biophysica Acta-Biomembranes. 2015;1848:60-66.
  4. Korytowski W, Wawak K, Pabisz P, Chadwick  A, Sahoo D, Schmitt J.C, Girotti AW. Impairment of macrophage reverse cholesterol transport by StAR-dependent cholesterol hydroperoxide trafficking: Implications for atherogenesis under oxidative stress. Arterioscler Thromb Vasc Biol. 2015;35(10):2104-13.
  5. Wolnicka-Glubisz A, Nogal  K, Zadlo A, Plonka P.M. Curcumin does not switch melanin synthesis towards pheomelanin in B16F10 cells. Archives of Dermatological Research. 2015;307:89-98.
  6. Wolnicka-Glubisz A, Strickland FM, Wielgus A, Anver M, Merlino G, De Fabo EC, Noonan FP. A melanin-independent interaction between Mc1r and Met signaling pathways is required for HGF-dependent melanoma. International Journal of Cancer. 2015;136:752-760.
  7. Korytowski W, Pilat A, Schmitt JC, Girotti AW. Deleterious cholesterol hydroperoxide trafficking in steroidogenic acute regulatory (StAR) protein-expressing MA-10 Leydig cells: implications for oxidative stress-impaired steroidogenesis. J Biol Chem. 2013;288(16):11509-19.
  8. Pilat A, Herrnreiter AM, Skumatz CM, Sarna T, Burke JM. Oxidative stress increases HO-1 expression in ARPE-19 cells, but melanosomes suppress the increase when light is the stressor. Invest Ophthalmol Vis Sci. 2013;54(1):47-56.
  9. Beattie JR, Pawlak AM, Boulton ME, Zhang J, Monnier VM, McGarvey JJ, Stitt AW. Multiplex analysis of age-related protein and lipid modifications in human Bruch's membrane. FASEB J. 2010;24(12):4816-24.
  10. Subczynski W.K, Wisniewska A, Widomska J. Location of macular xanthophylls in the most vulnerable regions of photoreceptor outer-segment membranes. Arch. Biochem. Biophys. 2010;504:61-6.
     

Batchelor/master thesis topics

Laboratory for Cancer Radiospectroscopy and Radiobiology:

  • Radiobiology and photodynamic therapy at the cell and organism level
  • Application of EPR in biology and medicine
  • Hypoxia in cancer
  • Melanin and its properties
  • Cancer heterogeneity
  • Hair cycle regulation
  • Biology of nitric oxide
  • Slime molds as alternate model organisms
  • Models of tumor growth, biocomputing and biosemiotics

Laboratory of Photobiophysics:

Requirements for candidates

Laboratory for Cancer Radiospectroscopy and Radiobiology:
knowledge in cell biology, interest in cancer problems, good English, computer modelling (basic)

Laboratory of Photobiophysics:
good English