Department of General Biochemistry

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Head

Prof. Jolanta Jura (maiden name: Kwiatkowska), PhD, DSc
room: B125 (3.0.29), phone: +48 12 664 63 59, e-mail: jolanta.jura@uj.edu.pl

Staff

Jerzy Kotlinowski, PhD
room: C144 (2.0.32), phone: +48 12 664 61 39, e-mail: j.kotlinowski@uj.edu.pl

Katarzyna Miękus, PhD
room: A116 (4.0.19), phone: +48 12 664 65 23, e-mail: katarzyna.miekus@uj.edu.pl

Agata Lichawska-Cieślar, PhD
room: C144 (2.0.32), phone: +48 12 664 61 39, e-mail: agata.lichawska@uj.edu.pl

Natalia Kordulewska, PhD
room:  A116 (4.0.19), phone: +48 12 664 65 23

Tomasz Herjan, PhD
e-mail: tomasz.herjan@uj.edu.pl 

Monika Dziendziel, MSc
room: A117 (4.0.20), phone: +48 12 664 65 31, e-mail: monika.dziendziel@uj.edu.pl

Barbara Strzelbicka, MSc
room: A117 (4.0.20), phone: +48 12 664 65 31, e-mail: barbara.strzelbicka@uj.edu.pl

Beata Bugara, Msc
room: C145 (2.0.33), phone: +48 12 664 62 18, e-mail: beata.bugara@doctoral.uj.edu.pl

Mateusz Wilamowski, Msc
room: C145 (2.0.33), phone: +48 12 664 62 18, e-mail: mateusz.wilamowski@uj.edu.pl

PhD students

Anna Adamczyk, room: C145 (2.0.33), phone: +48 12 664 62 18
Piotr Konieczny, room: C145 (2.0.33), phone: +48 12 664 62 18
Magdalena Łośko, room: C145 (2.0.33), phone: +48 12 664 62 18
Paulina Marona, room: C145 (2.0.33), phone: +48 12 664 62 18
Magdalena Kukla-Bartoszek, room: C145 (2.0.33), phone: +48 12 664 62 18
Natalia Pydyn, room: C145 (2.0.33), phone: +48 12 664 62 18
Judyta Górka, room: C145 (2.0.33), phone: +48 12 664 62 18
Róża Pietrzycka, room: C145 (2.0.33), phone: +48 12 664 62 18

Research topics

  • Regulation of inflammatory processes
  • Activation of signaling pathways
  • Role of  MCPIP1 in adipogenesis
  • Structural analysis of MCPIP1
  • Molecular background of renal cell carcinoma: genetic and molecular studies
  • Cell differentiation, inflammation associated with skin disorders
  • Role of inflammation in etiology of metabolic diseases
  • miRNA/lncRNA/mRNA profile in selected disorders/pathogenic states

Methods

  • Culture of primary cells and cell lines
  • Gene transfer techniques: transfection, transduction
  • siRNA strategy
  • Reporter gene assays
  • PCR, real-time PCR, RACE, differential display
  • Western Blot, immunoprecipitation assay, immunohistochemical stainings
  • Molecular cloning – plasmid and viral vectors
  • Colorimetric, fluorimetric and luminometric tests: proliferation, migration, viability, apoptosis
  • In vivo models: tumor inoculation, conditional knockout mice, transgenic pig overexpressing MCPIP1 in adipose tissue

Current projects

  1. Tomasz Herjan: The function of the RNA-binding protein HuR in IL-17-associated severe astma. (2017-2020). SONATA 11, National Science Centre (NCN).
  2. Jolanta Jura: Role of MCPIP1 protein in epidermis physiology and pathophysiology.  (2017-2020). OPUS 12, National Science Centre (NCN).
  3. Jerzy Kotlinowski: MCPIP1 as an important regulator of development and progression of non-alcoholic fatty liver disease. (2016-2019). Sonata 10, National Science Centre (NCN).
  4. Katarzyna Miękus: The role of MCPIP1 protein in growth, progression and vascularisation of clear cell renal cell carcinoma. (2016-2019). Opus 10, National Science Centre (NCN).
  5. Jolanta Jura: Structure analysis and identification of templates degraded by Regnase-1. (2016-2018). Opus 9, National Science Centre (NCN).
  6. Piotr Konieczny: The role of MCPIP1 Rnase in proliferation and differentiation of primary human keratinocytes. (2015-2018). Preludium 7, National Science Centre (NCN).
  7. Jolanta Jura: Identification of transcripts degraded by regnase-1 and their significance in adipogenesis. (2014-2018). Opus 6, National Science Centre (NCN).
  8. Katarzyna Miękus: Molecular analysis of clear cell renal cell carcinoma progression in the context of the search for predictive factors prior to treatment. (2014-2018). Sonata 5, National Science Centre (NCN).

Selected publications

  1. Losko M, Lichawska-Cieslar A, Kulecka M, Paziewska A, Rumienczyk I, Mikula M, Jura J. Ectopic overexpression of MCPIP1 impairs adipogenesis by modulating microRNAs. Biochim Biophys Acta2017; Sep 19. pii: S0167-4889(17)30253-7. 
  2. Marona P, Górka J, Mazurek Z, Wilk W, Rys J, Majka M, Jura J, Miekus K. MCPIP1 Downregulation in Clear Cell Renal Cell Carcinoma Promotes Vascularization and Metastatic Progression. Cancer Res2017; Sep 15;77(18):4905-4920. 
  3. Lipert B, Wilamowski M, Gorecki A, Jura J. MCPIP1, alias Regnase-1 binds and cleaves mRNA of C/EBPβ. PLoS One2017; Mar 22;12(3):e0174381. 
  4. Ligeza J, Marona P, Gach N, Lipert B, Miekus K, Wilk W, Jaszczynski J, Stelmach A, Loboda A, Dulak J, Branicki W, Rys J, Jura J. MCPIP1 contributes to clear cell renal cell carcinomas development. Angiogenesis2017; Aug;20(3):325-340. 
  5. Ruiz-Romeu E, Ferran M, Giménez-Arnau A, Bugara B, Lipert B, Jura J, Florencia EF, Prens EP, Celada A, Pujol RM, Santamaria-Babi LF. MCPIP1 RNase is aberrantly distributed in psoriatic epidermis and rapidly induced by IL-17A. J Invest Dermatol2016; S0022-202X(16)31153-8.
  6. Skrzypek K, Kusienicka A, Szewczyk B, Adamus T, Lukasiewicz E, Miekus K, Majka M. Constitutive activation of MET signaling impairs myogenic differentiation of rhabdomyosarcoma and promotes its development and progression. Oncotarget2015;13;6(31):31378-98.
  7. Pośpiech E, Ligęza J, Wilk W, Gołas A, Jaszczyński J, Stelmach A, Ryś J, Blecharczyk A, Wojas-Pelc A, Jura J, Branicki W. Variants of SCARB1 and VDR Involved in Complex Genetic Interactions May Be Implicated in the Genetic Susceptibility to Clear Cell Renal Cell Carcinoma. Biomed Res Int2015;2015:860405.
  8. Lipert B, Wegrzyn P, Sell H, Eckel J, Winiarski M, Budzynski A, Matlok M, Kotlinowski J, Ramage L, Malecki M, Wilk W, Mitus J, Jura J. Monocyte chemoattractant protein-induced protein 1 impairs adipogenesis in 3T3-L1 cells. Biochim Biophys Acta2014;1843(4):780-8.
  9. Konieczny P, Goralczyk AG, Szmyd R, Skalniak L, Koziel J, Filon FL, Crosera M, Cierniak A, Zuba-Surma EK, Borowczyk J, Laczna E, Drukala J, Pyza E, Semik D, Woznicka O, Klein A, Jura J. Effects triggered by platinum nanoparticles on primary keratinocytes. Int J Nanomedicine2013;8:3963-75.
  10. Skalniak L, Koj A, Jura J. Proteasome inhibitor MG-132 induces MCPIP1 expression. FEBS J2013;280(11):2665-74.

Batchelor/master thesis topics

  • Expression alterations of chosen genes in regulation of inflammatory processes
  • Regulation alterations of mRNA stability, coding for cytokines and growth factors, in cancer, diabetes and metabolic syndrome pathogenesis  
  • MCPIP as an important regulator of inflammatory state
  • Role of protein kinases in mitogenic signal transmission
  • Nutrients impact on level of endogenous oxidative DNA damage

Requirements for candidates

Basic knowledge in biochemistry and molecular biology