Department of Physical Biochemistry

Head

Prof Marta Dziedzicka–Wasylewska, PhD, DSc
room: C021 (2.01.22), phone: +48 12 664 61 22, e-mail: marta.dziedzicka-wasylewska@uj.edu.pl

Staff

Sylwia Kędracka-Krok, PhD, DSc
proom: C020 (2.01.21), phone: +48 12 664 61 48, e-mail: sylwia.kedracka-krok@uj.edu.pl

Piotr Bonarek, PhD
room: C023 (2.01.24), phone: +48 12 664 61 51, e-mail: piotr.bonarek@uj.edu.pl

Andrzej Górecki, PhD
room: C023 (2.01.24), phone: +48 12 664 61 51, e-mail: andrzej.gorecki@uj.edu.pl

Agnieszka Polit, PhD
room: C019 (2.01.20), phone: +48 12 664 61 56, e-mail: a.polit@uj.edu.pl

Ewelina Fic, PhD
room: C020 (2.01.21), phone: +48 12 664 61 48, e-mail: ewelina.fic@uj.edu.pl

Sylwia Łukasiewicz, PhD
room: C022 (2.01.23), phone: +48 12 664 61 34, e-mail: sylwia.lukasiewicz@uj.edu.pl

Ewa Błasiak, PhD
room: C019 (2.01.20), phone: +48 12 664 61 56, e-mail: ewa.blasiak@uj.edu.pl

Małgorzata Figiel, PhD
room: C022 (2.01.23), phone: +48 12 664 61 34, e-mail: m.figiel@uj.edu.pl

Anna Chmielińska, MSc
room: C024 (2.01.25), phone: +48 12 664 61 54, e-mail: anna.chmielinska@uj.edu.pl

Paweł Mystek, MSc
room: C024 (2.01.25), phone: +48 12 664 61 54, e-mail: pawel.mystek@uj.edu.pl

PhD students

Anna Chmielińska, room: C024 (2.01.25), phone: +48 12 664 61 54
Paweł Mystek, room: C024 (2.01.25), phone: +48 12 664 61 54
Bożena Skupień-Rabian, room: C025 (2.01.26), phone: +48 12 664 61 55
Bianka Świderska, room: C025 (2.01.26), phone: +48 12 664 61 55
Beata Rysiewicz, room: C024 (2.01.25), phone: +48 12 664 61 54
Julia Łakomska, room: C025 (2.01.26), phone: + 48 12 664 61 55

Research topics

  • Molecular mechanisms of eukariotic genes transcription activation by Yin Yang 2 (YY2)
  • Interaction of YY1 and YY1 mutants with molecular partners and DNA promoter sequences
  • Ligand binding specificity of beta lactoglobulin
  • Mechanism of selected drugs binding by beta lactoglobulin
  • Mechanisms of GPCRs and G proteins interactions
  • Mechanisms of GPCRs and G proteins interactions with lipid membranes
  • Efficient production and purification of GPCRs
  • Role of homo- and hetero oligomerization of GPCRs
  • Nanoparticles selectively recognizing GPCR oligomers
  • Influence of antidepressants and antipsychotic drugs on proteome
  • Proteomic characterization of T cells subpopulations isolated from blood of depressive  patients

Methods and specialistic equipment

  • Molecular biology techniques: cloning, mutagenesis
  • Recombinant protein production and purification: New Brunswick Innova 43R, Amersham, ÄktaExplorer
  • Confocal microscopy (FRET-FLIM, FRAP)
  • Isothermal and differential microcalorimetry (ITC, DSC): MicroCal, CSC 6100
  • Spectroscopic methods (circular dichroism, steady-state and time resolved fluorescence, fluorescence anisotropy, FRET): Jasco J-170, Horiba, system 5000U
  • Fast kinetics techniques (T-jump, stopped-flow): Applied Photophysics SX-17 MW, Hi-Tech Scientific PTJ-64
  • Two-dimensional electrophoresis (2DE): GE Healthcare Ettan DALTsix
  • Differential in gel electrophoresis (DIGE)
  • Mass spectrometry coupled with liquid chromatography (LC-MS/MS) in proteomics

Current projects

  1. Agnieszka Polit: The effect of membrane domains on the G protein-lipid interaction (2017-2020). OPUS 12, National Science Centre (NCN).
  2. Małgorzata Figiel: DNA sequences recognized by YY1 and YY2 transcription factors - comparative analysis (2017-18). MINIATURA 1, National Science Centre (NCN).
  3. Ewelina Fic: Optimization the strategy of tandem affinity purification (TAP) for the efficient isolation and identification of protein complexes involved in molecular mechanism of depression (2017-2018). MINIATURA 1, National Science Centre (NCN).
  4. Bożena Skupień-Rabian: Influence of selected antipsychotic drugs on the nuclear proteome in rat brain (2018). ETIUDA 5, National Science Centre (NCN).
  5. Sylwia Kędracka-Krok: Evaluation of the role of subpopulations of T lymphocytes (Treg: CD4+CD25+, CD4+CD25- i Teff: Th2) in depression using proteomic analysis of blood of patients (2013-2016, prolonged to 2017). OPUS 3, National Science Centre (NCN).

Selected publications

  1. Nieborak A, Górecki A. Significance of the pathogenic mutation T372R in the Yin Yang 1 protein interaction with DNA - thermodynamic studies. FEBS Lett. 2016;590(6):838-47.
  2. Łukasiewicz S, Szczepanowicz K, Podgórna K, Błasiak E, Majeed N, Ogren SO, Nowak W, Warszyński P, Dziedzicka-Wasylewska M. Encapsulation of clozapine in polymeric nanocapsules and its biological effects. Colloids Surf B Biointerfaces. 2016;140:342-52.
  3. Łukasiewicz S, Błasiak E, Szafran-Pilch K, Dziedzicka-Wasylewska M. Dopamine D2 and serotonin 5-HT1A receptor interaction in the context of the effects of antipsychotics – in vitro studies. J Neurochem. 2016;137(4):549-60.
  4. Łukasiewicz S, Szczepanowicz K, Błasiak E, Dziedzicka-Wasylewska M. Biocompatible Polymeric Nanoparticles as Promising Candidates for Drug Delivery. Langmuir. 2015;31(23):6415-25.
  5. Górecki A, Bonarek P, Górka AK, Figiel M, Wilamowski M, Dziedzicka-Wasylewska M. Intrinsic disorder of human Yin Yang 1 protein. Proteins. 2015;83(7):1284-96.
  6. Mystek P, Tworzydło M, Dziedzicka-Wasylewska M, Polit A. New insights into the model of dopamine D1 receptor and G-proteins interactions. Biochim Biophys Acta. 2015 ;1853(3):594-603.
  7. Kędracka-Krok S, Swiderska B, Jankowska U, Skupień-Rabian B, Solich J, Buczak K, Dziedzicka-Wasylewska M. Clozapine influences cytoskeleton structure and calcium homeostasis in rat cerebral cortex and has a different proteomic profile than risperidone. J Neurochem. 2015;132(6):657-76.
  8. Loch JI, Bonarek P, Polit A, Jabłoński M, Czub M, Ye X, Lewiński K. β-Lactoglobulin interactions with local anaesthetic drugs – Crystallographic and calorimetric studies. Int J Biol Macromol. 2015;80:87-94.
  9. Gołębiowski FM, Górecki A, Bonarek P, Rapala-Kozik M, Kozik A, Dziedzicka-Wasylewska M. An investigation of the affinities, specificity and kinetics involved in the interaction between the Yin Yang 1 transcription factor and DNA. FEBS J. 2012;279(17):3147-58.
  10. Dziedzicka-Wasylewska M, Faron-Górecka A, Andrecka J, Polit A, Kuśmider M, Wasylewski Z. Fluorescence studies reveal heterodimerization of dopamine D1 and D2 receptors in the plasma membrane. Biochemistry. 2006;45(29): 8751-9.

Requirements for candidates

  • Basic knowledge on structural biochemistry
  • Basic knowledge on molecular biology and  protein engineering
  • Basic knowledge on protein production and purification
  • Basic knowledge on proteomics