Department of Cell Biochemistry

Head

Prof. Joanna Bereta, PhD, DSc
room: B122 (3.0.26), phone: +48 12 664 63 56

Staff

Aneta Kasza, PhD, DSc
room: A114 (4.0.17), phone: +48 12 664 65 21, e-mail: aneta.kasza@uj.edu.pl

Monika Bzowska, PhD
room: B123 (3.0.27), phone: +48 12 664 63 88, e-mail: monika.bzowska@uj.edu.pl

Krystyna Stalińska, PhD
room: B119 (3.0.23), phone: +48 12 664 61 41, e-mail: krystyna.stalinska@uj.edu.pl

Dominik Czaplicki, PhD
room: B118 (3.0.22), phone: +48 12 664 63 41, e-mail: dominik.czaplicki@uj.edu.pl

Renata Mężyk-Kopeć, PhD
room:  B123 (3.0.27), phone: +48 12 664 63 38, e-mail: renata.mezyk-kopec@uj.edu.pl

Anna Stachurska, PhD
room: B118 (3.0.22), phone: +48 12 664 63 41, e-mail: anna.stachurska@uj.edu.pl

Mateusz Wawro, PhD
room: C142 (2.0.34), phone: +48 12 664 64 00, e-mail: mateusz.wawro@uj.edu.pl

Urszula Tuleja, MSc
room: B120 (3.0.24), phone: +48 12 664 63 57, e-mail: urszula.tuleja@uj.edu.pl

Edyta Żyła, MSc
room: C138 (3.0.14), phone: +48 12 664 61 41, e-mail: edyta.zyla@doctoral.uj.edu.pl

PhD students

Maria Czarnek, room: B123 (3.0.27), phone: +48 12 664 63 88
Jarosław Jucha, room: B123 (3.0.27), phone: +48 12 664 63 88
Alicja Karabasz, room: B129 (3.0.21), phone: +48 12 664 64 04
Tomasz Klaus, room: B130 (3.0.11), phone: +48 12 664 64 05
Karolina Ossysek, room: B118 (3.0.22), phone: +48 12 664 63 41
Aleksandra Soleckaroom: C142 (2.0.34), phone: +48 12 664 64 00
Weronika Sowińskaroom: C142 (2.0.34), phone: +48 12 664 64 00
Barbara Wyroba, room: B118 (3.0.22), phone: +48 12 664 63 41
Edyta Żyłaroom: C138 (3.0.14), phone: +48 12 664 61 41

Research topics

  • Regulation of the expression of genes involved in inflammation and cancer
  • Influence of cytokines and growth factors on the expression of selected genes
  • Functions, regulation of expression, and the mechanism of action of sheddases of the ADAM family
  • Mechanisms involved in the regulation of transcript stability
  • Genetic modifications of Salmonella typhimurium strains for improvement of their antitumor potential
  • Generation and characterization of monoclonal antibodies specific to selected proteins – their applications in research, diagnostics and therapies
  • Structure, stability, and functions of mouse IgG3 and IgM immunoglobulins in the light of their natural roles and potential use in diagnostics and therapies
  • Nanoparticle-based drug delivery systems

Methods and specialistic equipment

  • Methods of molecular biology: PCR, RT-PCR, qRT-PCR, EMSA, restriction analysis, genetic engineering, generation of genetic constructs, directed mutagenesis, gene silencing with siRNA, transient and stable transfection of eukaryotic cells
  • Genome editing tools: CRISPR/CAS9, TALEN
  • Protein expression in prokaryotic and eukaryotic systems
  • Generation of monoclonal antibodies using cell fusion (hybridoma)- and phage display methodologies
  • Modifications of proteins including antibodies
  • Immunochemical techniques: ELISA, Western blotting, flow cytometry, immunofluorescence staining
  • Analysis of enzyme activities (e.g. proteases, SOD, iNOS, LDH, other oxidoreductases)
  • Microbiological methods, e.g. analysis of infectivity and cytotoxic potential of Salmonella strains 
  • Analysis of cell metabolism in cell cultures
  • In vivo studies of nanoparticle-based drug delivery and cancer treatments in mouse models

Current projects

  1. Joanna Bereta: ADAM17 as a key protein in cellular response to proinflammatory mediators (2017-2020). OPUS 11, National Science Centre (NCN).
  2. Aneta Kasza: PAnalysis of the inflammatory role of MCPIP2 using the CRISPR/dCas9-FokI system. Identification of MCPIP2 substrates and interacting proteins, as well as factors controlling MCPIP2 expression. (2015-2018). National Science Centre (NCN).
  3. Alicja Karabasz: Analysis of toxicity and biodistribution of polyelectrolyte nanocapsules. (2014-2018). DIAMOND GRANT, Ministry of Science and Higher Education (MNiSW).

Selected publications

  1. SmartFlares fail to reflect their target transcripts levels. Czarnek M, Bereta J. Sci Rep2017 Sep 15;7(1):11682. 
  2. Intact NYN/PIN-Like Domain is Crucial for the Degradation of Inflammation-Related Transcripts by ZC3H12D. Wawro M, Kochan J, Krzanik S, Jura J, Kasza A. J Cell Biochem2017 Mar;118(3):487-498. 
  3. The CRISPR-Cas system - from bacterial immunity to genome engineering. Postepy Hig Med Dosw (Online). Czarnek M, Bereta J. 2016 Sep 1;70(0):901-16. Review.
  4. Agglutinating mouse IgG3 compares favourably with IgMs in typing of the blood group B antigen: Functionality and stability studies. Klaus T, Bzowska M, Kulesza M, Kabat AM, Jemioła-Rzemińska M, Czaplicki D, Makuch K, Jucha J, Karabasz A, Bereta J. Sci Rep2016 Aug 3;6:30938. 
  5. Pegylated polyelectrolyte nanoparticles containing paclitaxel as a promising candidate for drug carriers for passive targeting. Szczepanowicz K, Bzowska M, Kruk T, Karabasz A, Bereta J, Warszynski P. Colloids Surf B Biointerfaces2016 Jul 1;143:463-71.
  6. IF-combined smRNA FISH reveals interaction of MCPIP1 protein with IER3 mRNA. Kochan J, Wawro M, Kasza A. Biol Open2016 Jun 2. pii: bio.018010.
  7. A new expression vector facilitating production and functional analysis of scFv antibody fragments selected from Tomlinson I+J phagemid libraries. Ossysek K, Uchański T, Kulesza M, Bzowska M, Klaus T, Woś K, Madej M, Bereta J. Immunol Lett2015 Oct;167(2):95-102.
  8. Simultaneous detection of mRNA and protein in single cells using immunofluorescence-combined single-molecule RNA FISH. Kochan J, Wawro M, Kasza A. Biotechniques2015 Oct 1;59(4):209-12.
  9. ADAM17 Promotes Motility, Invasion, and Sprouting of Lymphatic Endothelial Cells.Mężyk-Kopeć R, Wyroba B, Stalińska K, Próchnicki T, Wiatrowska K, Kilarski WW, Swartz MA, Bereta J. PLoS One2015 Jul 15;10(7):e0132661.
  10. Transcription factor Elk-1 participates in the interleukin-1beta-dependent regulation of expression of immediate early response gene 3 (IER3).Kochan J, Wawro M, Kolka A, Maczuga P, Kasza A. Cytokine2014 Dec;70(2):120-5

Batchelor/master thesis topics

The topics for B.Sc. and M.Sc. thesis are directly related to the current research lines that are pursued at our Department.

Requirements for candidates

The students are expected to have a sound background in biochemistry and molecular biology, as well as good language skills in English.